The new rotovap must be cleaned after use.
The new rotovap is mainly a common laboratory equipment composed of a motor, a distillation flask, a heating pot, a condenser, and other parts. The rotovap is mainly used for continuous distillation of a large amount of volatile solvents under reduced pressure. Especially for the concentration of the extract and the distillation of the receiving liquid during chromatographic separation, the reaction products can be separated and purified.
Working principle:
Through electronic control, the flask is rotated at a constant speed at a suitable speed to increase the evaporation area. The evaporation flask is placed in a negative pressure state through a vacuum pump. The evaporation flask is placed in a water bath pot for constant temperature heating while rotating, and the solution in the flask is heated and diffused in the rotating flask under negative pressure. The new rotovap system can seal and reduce the pressure to 400-600 mmHg; use a heating bath to heat the solvent in the distillation flask, and the heating temperature can be close to the boiling point of the solvent; it can also rotate at a speed of 50-160 rpm to form a thin film of the solvent and increase the evaporation area. In addition, under the action of the cooler, the hot vapor can be quickly liquefied to accelerate the evaporation rate.
Cleaning of new rotovap:
After using the instrument, wipe the interface of the rotovap with purified water using a dust-free cloth. Follow the evaporation procedure and rinse the rotovap repeatedly with water until there is no residue left, and use a suction bottle to extract the residual liquid in the evaporation bottle. Turn off the vacuum and clean the outside of the rotovap with purified water. Seal all the openings with plastic bags, hang a cleaning sign, and keep it ready for use. If the rotovap is found to be damaged or faulty, notify the maintenance personnel immediately to replace it.
Precautions:
1. Start-up sequence: During operation, pay attention to the principle of vacuuming (about 0.03 MPa or more) before rotating to prevent the distillation flask from sliding due to gravity. Stop sequence: Stop the rotation first, hold the distillation flask by hand, empty the air intake, and stop the vacuum pump when the vacuum degree drops to about 0.04 MPa to prevent the distillation flask from falling off and back-inhalation.
2. Before turning on the constant temperature bath, clean it and add water to the designated position first. Dry burning without water is not allowed.
3. Before installing each interface, sealing surface, sealing ring, and joint, a layer of lubricating vacuum grease needs to be applied. If the vacuum degree is too low, the air tightness of the joints of the vacuum tube, the joints of the condenser, the joints of the gas guide bottle and the condenser and the host, and the joints of the host and the rotovap should also be checked.
4. When concentrating substances that are sensitive to air, first pass nitrogen from the exhaust port to exhaust the air in the rotovap, and then connect the sample for rotary evaporation. After evaporation, release nitrogen to increase the pressure, then turn off the pump, and then remove the sample bottle and seal it.
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