How is recirculating chiller bath used in biochemistry experiments?

In biochemical experiments, recirculating chiller bath is used to maintain a constant low temperature for a variety of applications. Here are step-by-step instructions on how to use it:

1.  Preparation  : Ensure that the cooler is placed on a level surface and has adequate ventilation and power supply.

2.  Connection  : Use appropriate hoses to connect the recirculating chiller bath to the equipment or process that requires cooling for coolant circulation.

3.  Power on  : Plug the cooler into the power outlet and turn it on.

4.  Temperature setting  : Adjust the temperature control to the required low temperature, usually set according to the experimental requirements.

5.  Cycle  : Once the set temperature is reached, the recirculating chiller bath will circulate the coolant through the connected system to maintain the temperature.

6.  Monitoring  : Continuously monitor the temperature to ensure that the temperature remains stable throughout the experiment.

7. Safety: Always follow safety procedures, including wearing appropriate protective equipment and ensuring that coolers are not overloaded.

8.  Cleaning  : After the end of the experiment, disconnect the hose, close the recirculating chiller bath, and clean the system as needed to prevent contamination in future use.

By maintaining a stable temperature, recirculating chiller bath is essential for experiments that are sensitive to temperature changes, such as enzyme reactions or cell cultures.